Pretty much the only way to be sure you remove all malware is to back up your personal files, then wipe the computer and do a clean re-install of the operating system and all your applications (including deleting and re-creating the boot partition). Anything short of that, you'll just never know if you got everything. You've got to have all the installation media (CD's, DVD's, etc.) for the OS and your applications (or if you got one when you bought the PC, a so-called "restore disk" that puts the computer back the way it was when you bought it).
This is not at all fun. Depending on how many applications you have, it can take anywhere from several hours to several days to get it all back the way it was. If you hire someone to do it for you, be sure they do a clean re-install and not just run some malware-removal tool that claims to be able to remove everything. There are some pretty good ones, but nothing short of the clean re-install can deliver reasonable confidence that you got everything. Anyone who does this for a living has my utmost respect. Its hard work.
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