On a period, single action weapon, opening the gate and rotating to each cylinder will ONLY show how many "rounds" are chambered (or not). Doing so will (most likely) not verify whether the "round" is a blank or live ammo.
I say most likely as it is a possibility there could be different identification/marking of some sort to the primer view of the "round"; if not there is no way to tell looking at the primer end of the "round".
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