There's the alt-med center:
HealthCare Partners Family Medicine
The doctor there is an MD, not a DO, but they specialize in holistic and alternative medicine. They're on 441 across the street from the Villages/UF Spanish Plaines/Name_of_the_Month Hospital.
There's a DO office with Dr. Karl Mueller and Dr. Connie Mercer at the Orlando Health Medical Group, also on 441, across from the Plaza Grande strip-mall. They're in the same area as Tire Choice, just south of the golf cart bridge into the Historic Section.